<p>We present the first representation of grass pollen in a 3D dispersion model anywhere in Australia, tested using observations from eight counting sites in Victoria. The region's population has high rates of allergic rhinitis and asthma, and this has been linked to the high incidence of grass pollen allergy. Despite this, grass pollen dispersion in the Australian atmosphere has not been studied previously, and its source strength is untested. We describe ten pollen emission source methodologies examining the strengths of different immediate and seasonal timing functions, and spatial distribution of the sources. The timing function assumes a smooth seasonal term, modulated by an hourly meteorological function. A simple Gaussian representation of the pollen season worked well (average r = 0.54), but lacks the spatial and temporal variation that the satellite-derived Enhanced Vegetation Index (EVI) data can provide. However poor results were obtained using the EVI gradient (average r = 0.35), which gives the timing when grass turns from maximum greenness to a drying and flowering period; this is due to the greater spatial and temporal variability from this combined spatial and seasonal term. Better results were obtained using statistical methods that combine elements of the EVI dataset, a smooth seasonal term and instantaneous variation based on historical grass pollen observations (average r = 0.69). The seasonal magnitude is inferred from the maximum winter-time EVI, while the timing of the peak of the season was based on the day of the year when the EVI falls to 0.05 below its winter maximum. Measurements are vital to monitor changes in the pollen season, and the new pollen measurement sites in the Victorian network should be maintained.</p>